The competition begins in New Brighton, Merseyside, where Andy Johnson and Dave Owen run Dunsandles Guesthouse.
The guests then head to the waterways of Lancashire to board the Lady Teal Hotel Boat owned by Nick and Gina Mead.
The third visit is to Brock Burgius' York House B&B in Tetbury, Gloucestershire.
Finally the B&Bers visit Heyford House in Lower Heyford, Oxfordshire run by Sonya and Leo Brooke-Little.