Teenage photographer Peter Parker is sent by the Daily Bugle to investigate a series of mystery lights in a wooded area outside of the city, when a sudden landslide forces him to swerve off the road and land in a tree.

On a blistering hot afternoon, Peter is walking to the house of a brilliant scientist, Doctor Smartyr, when suddenly he notices that the sidewalk, and most of the neighborhood, is turning to ice. Hearing desperate cries for help from Smartyr's house, Peter changes into Spider-Man and breaks through a laboratory window to find the frightened scientist being menaced by a towering ice creature.

Peter flies to Florida to find and photograph the Lizard Man. Spider-Man meets Dr. Curtis Connor's family. He is pulled underwater by the Lizard Man, but escapes.

Spider-Man is set up numerous times by Electro's robberies.

Spider-Man has been witnessed committing a robbery of the Midtown Museum.

The Vulture uses a sonic device in his head mask to control an army of vultures. After the Vulture beats Spider-Man, he holds the city up for a $2,000,000 ransom and J. Jonah Jameson blames Spider-Man.

An inventor named Henry Smythe reveals his plans to capture Spider-Man with a robot and get paid by J. Jonah Jameson for his efforts.

Dr. Stillwell creates the Scorpion on J. Jonah Jameson's behalf to capture Spider-Man. The Scorpion attacks Spider-Man, who escapes. However, the Scorpion's evil nature takes over as his strength increases which causes him to go after Jameson.

As Spider-Man examines the Goliath Diamond, the Sandman appears and swipes the diamond, with Spider-Man getting the blame as he is seen by the guards.

Spider-Man witnesses, and photographs, a giant metal-eating robot in Central Park.

The Green Goblin swipes Grandini the Mystic's witchcraft book, hoping to control demons of the Underworld.

Electro escapes prison, before Spider-Man can stop him, using a metal kite during an electrical storm to renew his powers.

Spider-Man faces off against Parafino, a wax museum owner who plans to make Spider-Man into his latest exhibit.

Spider-Man is keeping an eye on a train when the Rhino rams into it and steals the first component of a top secret weapon.

Someone has sent a one-eyed idol to J. Jonah Jameson. Jameson is hypnotized by the idol to steal his own money. Spider-Man finds the thief, but he escapes with the money.

Spider-Man attempts to halt the Phantom's activities only to be set up by him and his robot henchwoman Marie, who is disguised as a shop window mannequin.

Spider-Man saves a boat from Dr. Matto Magneto's attempt to crash it on the rocks by sabotaging a lighthouse.

Spider-Man gets into an embassy, sees the foreign visiting Prime Minister tied up and gagged, and fights someone impersonating the Prime Minister, but he is forced to retreat to find proof after being gassed by the imposter's cane and having his camera stolen.

Historic villains Blackbeard the Pirate, Jesse James, and the Executioner of Paris are committing robberies in New York City.

Miss Trubble, a book dealer obsessed with mythology, is owner of a magical chest from which she summons a succession of mythological figures, from centaurs to the Cyclops to Diana the Hunter-Goddess, to commit robberies of ancient artifacts on her behalf.

A scientist, Dr. Noah Boddy, renders himself invisible by means of a machine, then acts to avenge himself upon Jameson, who publically maligned his theory of invisibility.

Spider-Man battles an Arabian jewel thief, whose magical flute induces animals into attacking Spidey.

Reports of a legendary flying ghost ship, the Flying Dutchman, being sighted near Smuggler's Cove summon Spidey to the area, where his investigation into the phantom ship's appearance leads him to a cave in which he finds his sworn adversary, Mysterio, plotting with a pair of thugs.

When a Jekyll-and-Hyde poster comes to life at the soon-to-be-demolished Castle Theatre, Spidey visits the theatre and encounters mischievous Blackwell the Magician, who is trying to attract public attention to the theatre in hope of preventing its demolition.

Spider-Man's old enemy, the rampaging Rhino, is stealing gold bullion to mold an auric likeness of himself.

A bald-headed mastermind, the Plotter, employs two ridiculous criminals, Cowboy and Ox, to steal a blueprint to a missile.

Spidey chases the culprit in an attempted theft of jewels from a countess and is surprised to find that his opponent, dressed in a black Human Fly costume, also has the ability to scale walls and can cross thin wires between buildings.

Oil is being stolen in huge quantities by Dr. Von Schlick, a chemist villain garbed in a rubber, non-stick suit and armed with petroleum-based bubbles that he fires from his fingers.

The nefarious Vulture traps Jameson inside of a tower-clock and uses the well-informed Daily Bugle publisher as a source of information on the whereabouts of a visiting diamond merchant and the testing of military equipment—two prospective heists for the greedy bird-man.

Life-like and substantial shadows of beasts are projected in various locations in New York City by the Phantom's new Shadow-Scope glasses to cause panic and enable the Phantom to effect unconstrained bank and jewelry store robberies.